Afterwards, a hydroponic culture system was used to study their effect on plant growth at an advanced stage. phytofirmans, Gfp (green fluorescent protein) marked mutant (PsJN*) was used for bacteria quantification in roots, stems and leaves using Real-Time PCR. To ascertain the endophytic behaviour of P. An in vitro assay was used to evaluate their effects on acidification, amylolytic activity in seeds, and on the peroxidases activity and phenolic content in wheat seedlings. In this context, seed coating with thyme essential oil and the endophytic bacterium Paraburkholderia phytofirmans was performed on durum wheat seeds in order to investigate their effects on germination of seeds and seedling growth. The use of biostimulants in plants is among the novel and integrated approaches in plant protection to increase crop tolerance to multiple biotic and abiotic stresses.
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